Antibodies reactive with cell surface carbohydrates.

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Antibodies reactive with cell surface carbohydrates.

Normal and immune sera from various animal species were fractionated on columns of Sepharose covalently coupled with the glycoprotein fetuin. Elution of the material bound to fetuin yielded low but reproducible amounts of protein, ranging from 0.02 to 0.2% of the protein mass of the input sera. This material has been identified by immunoelectrophoresis in agar and by zone electrophoresis on cel...

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Antibodies reactive with cell surface carbohydrates were isolated from normal chicken serum and were found to be mitogenic for mouse splenic lymphocytes as assayed by both blast transformation and [3H]thymidine incorporation. The Fab' fragments of these carbohydrate-binding immunoglobulins were just as mitogenic as the divalent native antibody. Moreover, succinylated Fab' fragments, which proba...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1975

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.72.3.1127